RESUMO
During a survey of floricolous yeasts in Portugal, a basidiomycetous yeast representing a novel species in the genus Hannaella was isolated in Portugal from the flower of Lantana camara, an ornamental exotic species native to Central and South America. A combination of phylogenetic analyses of DNA barcode sequences used in yeast molecular systematics, namely the D1/D2 domain and the complete internal transcribed spacer (ITS) region supported the recognition of a new species of Hannaella, that we designate Hannaella floricola sp. nov. (ex-type strain PYCC 9191T=CBS 18097T). Although the assignment of the new species to the genus Hannaella was evident, the detection of its closest relatives appeared more problematic. Nevertheless, our analyses suggested that H. floricola sp. nov. belongs a clade that also includes H. coprosmae, H. oryzae and H. surugaensis, together four candidate novel species. In addition we provide the molecular identification of several unidentified strains whose D1/D2 and ITS sequences are available from GenBank.
Assuntos
Ascomicetos , Basidiomycota , Lantana , DNA Fúngico/genética , Lantana/genética , Filogenia , Portugal , DNA Espaçador Ribossômico/genética , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Técnicas de Tipagem Micológica , DNA Bacteriano/genética , Técnicas de Tipagem Bacteriana , Composição de Bases , Ácidos Graxos/química , Ascomicetos/genética , FloresRESUMO
Abstract The occurrence of Myxomycetes in Heliconia psittacorum L.f. inflorescences was researched within four conservation units located in Northeast Brazil, aiming at evaluating the occupation of this microhabitat in fragments of Atlantic Forest along an altitude between 30-750 m. Inflorescences attached to the plant were examined; dead flowers and bracts were collected to assemble moist chambers (368). Four families, four genera and 10 species were recorded. A preference was evidenced for a basic pH substrate and a predominance of calcareous species (5:1). The composition of the myxobiota in fragments pertaining to altitudes above 400 m was similar and differed significantly from the one found in fragments of lowland forests (<100 m). Physarum compressum and Arcyria cinerea are the most characteristic species of the studied myxobiota.
Resumo A ocorrência de mixomicetos em inflorescências de Heliconia psittacorum L.f. foi pesquisada em quatro unidades de conservação situadas no Nordeste do Brasil, visando avaliar a ocupação deste microhabitat em fragmentos de Floresta Atlântica, numa faixa altitudinal de 30-750 m. Foram examinadas partes mortas de inflorescências ainda presas à planta; flores e brácteas mortas foram colhidas para montagem de câmaras-úmidas (368). Foram registrados quatro famílias, quatro gêneros e 10 espécies. Evidenciou-se preferência para substrato com pH básico e predominância de espécies calcárias (5:1). A composição da mixobiota nos fragmentos de altitudes acima de 400 m foi semelhante e diferiu significativamente da encontrada nos fragmentos de florestas de terras baixas (<100 m). Physarum compressum e Arcyria cinerea são as espécies mais características da mixobiota estudada.
